Single Cell RNA-Sequencing in Arabidopsis Root Tissues

Droplet-based single-cell RNA-sequencing (scRNA-seq)Single-cell RNA-sequencing (scRNA-seq) empowers transcriptomicTranscriptomics profiling with an unprecedented resolution, facilitating insights into the cellular heterogeneity of tissues, developmental progressions, stress-response dynamics, and more at single-cellSingle cells level. In this chapter, we describe the experimental workflow of processing ArabidopsisArabidopsis rootRoots tissue into protoplastsProtoplasts and generating single-cellSingle cells transcriptomes. We also describe the general computational workflow of visualizing and utilizing scRNA-seqSingle cell RNA-seq (scRNA-seq) data. This protocol can be used as a starting point for establishing a scRNA-seqSingle cell RNA-seq (scRNA-seq) workflow.