Enhanced Fluorescence Imaging of Implants Based on Polyester Copolymers in Combination With MRI

ABSTRACT Nowadays, many biodegradable materials are offered for biomedical applications, but there are only a few in vivo methods for their detection and monitoring. In this work, implants based on biodegradable polyester copolymers were labeled with indocyanine green (ICG) for fluorescence imaging in combination with tissue optical clearing (TOC) and magnetic resonance imaging (MRI). The results include in vitro degradation modeling followed by in vivo imaging of copolymer samples that were subcutaneously implanted in BALB/c mice. TOC with 70% glycerol has been demonstrated to significantly improve sample visualization. The TOC efficiency parameter Q demonstrated the variability of effects correlating with the timing of follow‐up in the postimplantation period. It has been shown that nonhealing wounds, peri‐implantation inflammation, or fibrosis, confirmed by MRI, affect the effectiveness of TOC in the range from Q = −30% to 70%.