Roles of myeloid dendritic cells in the development of helicobacter felis-inducedgastritis in mice

nant negative MyD88 ,.',*as performed using respective adenovims vectors. RESULTS: The primary cultures ~creted large amounts of superoxide 1150 nmoVmg protein/ha, while the cell lines produced small amounts (2-3 nmoFmg pmteiru%) Among the Nox/Duox family, only Noxi iso[brm was expressed in the colonic epitheIiai cells tested Primary cultured ceils also expressed p22-phox, p67-phox, and racl, but cancer cell lines possessed p22phox and racl. Owrexpression of p67-phox failed to increase superoxide generation in Caco2 cells, but co-transtection of p67q)hox and p47-phox up-regulated the production 10.4bid None of cyiokines (IL-l-beta, TNF-alpha and [FN-gamma), grovah factors (EGF and TGF-beta), E. colt LPS, and PMA up-regulated the Noxl activity Salmondla [lagellin (FIIC) sumulated constitutively expressed TLR5 in Caco2 cells, phosphorylated TAKIFFA81, and increased superoxide production 2-{old Tins up-regulation was cancelled by dominant negative MyD88 DISCUSSION: Our results show that the cytosolic components (p67-phox ang4~r p47-phox) are required tnr full activation of the potent Noxl expressed in colonic epithelial cells Noxl expressed on surface mucous cells of the colon may play an important role in host epithelial cell-bacterial interactions for I:lost defmzse