Mutagenesis directed by phosphotriester analogues of oligonucleotides: A way to site‐specific mutagenesis in vivo

A new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site-specific mutagenic primers. The achieved yield of mutant clones was 2-3%.