假交替单胞菌
鹿角珊瑚
珊瑚
海洋噬菌体
生物
细菌
微生物学
作者
Ocky Karna Radjasa,Torben Martens,Hans-Peter Grossart,Thorsten Brinkhoff,Agus Sabdono,Meinhard Simon
标识
DOI:10.3923/jbs.2007.239.246
摘要
A coral-associated bacterium was successfully screened for secondary metabolite production based on biological activity and PCR amplification of the non-ribosomal peptide synthetase (NRPS) gene and was identified as closely related to Pseudoatteromonas tuteoviotacea based on its 16S rDNA. The bacterium was found to inhibit the growth of other coral-associated bacteria and pathogenic bacteria. To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptidc synthctases and revealed a high similarity to NosD (40% identity), a multifunctional peptide synthetase from Nostoc sp. G5V224 and NdaB (44% identity), a peptide synthetase module of Nodutaria spumigena. To estimate the possible role of secondary metabolites, analyses on the quantitative proportion of Pseudoatteromonas was carried out. The results revealed that Pseudottermonas group was indeed present within surfaces of coral Acropora sp
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