Determination of ursodeoxycholic acid,chenodeoxycholic acid,cholic acid and lithocholic acid in compound ursodeoxycholic acid capsules by RP-HPLC

Objective To establish a reversed phase high performance liquid chromatography( RP-HPLC) to determine ursodeoxycholic acid,chenodeoxycholic acid,cholic acid and lithocholic acid in compound ursodeoxycholic acid capsules. Methods Diamonsil C18chromatographic column( 200 mm × 4. 6 mm,5 μm) w as used in the study,mobile phase w as acetonitrile-0. 03 mol·L- 1of phosphate solution( V ∶ V = 40 ∶ 60),the flow rate w as 1. 4 mL·min- 1,the detection wavelength was set at 205 nm,the column temperature was 35 ℃. Results Ursodeoxycholic acid,chenodeoxycholic acid,cholic acid and lithocholic acid w ere completely separated w ithin 25 min. A good linear range of 0. 80- 200. 00 mg·L- 1for ursodeoxycholic acid,0. 45-110. 00 mg·L- 1for cholic acid,0. 30-70. 00 mg·L- 1for chenodeoxycholic acid and 0. 30- 80. 00 mg·L- 1 for lithocholic acid w ere show ed respectively. The average recoveries of ursodeoxycholic acid,cholic acid, chenodeoxycholic acid and lithocholic acid w ere 99. 7%( RSD = 1. 30%),99. 3%( RSD = 1. 47%),98. 7%( RSD =1. 87%) and 99. 1%( RSD =1. 30%) respectively( n =12). Conclusions This method can simultaneously determine ursodeoxycholic acid,chenodeoxycholic acid,cholic acid and lithocholic acid contents in compound ursodeoxycholic acid capsules. The method is simple,accurate and reliable,and can be applied for the quality control of the compound ursodeoxycholic acid capsule.