Dominant bacterial populations and Ralstonia aolanacearum-inhibiting bacteria were isolated from the root-distributing area and rhizosphere and non-rhizosphere of healthy tomato plants at their seedling, flowering, initial fruiting and full fruiting stages to study the dynamic changes in their quantity. In all the growth stages, the quantity of rhizospheric bacteria was larger than that in other regions of the soil and was larger on the root surface than in the (non-)rhizosphere. Dominant bacterial populations remained the same throughout the growth stages and in various regions of the soil, showing no obvious dynamic changes. Rhizespherical effect (R/S) of tomato was the highest at the full-fruiting stage. Sixteen strains that inhibited R. aolanacearum were obtained.