谷氨酸受体
荧光
生物物理学
青色
生物
色阶
化学
生物化学
光学
物理
受体
作者
Jonathan S Marvin,Benjamin Scholl,Dana E. Wilson,Kaspar Podgorski,Abbas Kazemipour,Johannes Alexander Müller,Susanne Schoch,Francisco José Urra Quiroz,Nelson Rebola,Huan Bao,Justin P. Little,Ariana N. Tkachuk,Edward Cai,Adam W. Hantman,Samuel S.‐H. Wang,Victor J. DePiero,Bart G. Borghuis,Edwin R. Chapman,Dirk Dietrich,David A. DiGregorio,David Fitzpatrick,Loren L. Looger
出处
期刊:Nature Methods
[Springer Nature]
日期:2018-10-30
卷期号:15 (11): 936-939
被引量:302
标识
DOI:10.1038/s41592-018-0171-3
摘要
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates. Variants of the genetically encoded sensor iGluSnFR extend the range of conditions under which glutamate neurotransmission can be visualized. In addition, chromatic variants of iGluSnFR improve compatibility with various illumination schemes.
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