计算生物学
协议(科学)
生物
基因组
异源的
蛋白质表达
多样性(控制论)
病毒学
计算机科学
遗传学
基因
人工智能
医学
病理
替代医学
作者
Jochen Scholz,Sabine Suppmann
出处
期刊:Methods in Enzymology
日期:2021-01-01
卷期号:: 171-190
被引量:2
标识
DOI:10.1016/bs.mie.2021.06.015
摘要
Baculovirus-insect cell expression (BEV) has become one of the most widely used eukaryotic systems for heterologous protein expression. The combination of engineered baculovirus genomes together with a variety of compatible vectors, robust insect cell lines, serum-free media and commercial kits have made it a standard workhorse in many "non-virology-expert" laboratories. Despite these significant improvements, the BEV system still has major drawbacks, primarily the time required to amplify recombinant virus and its inherent instability. Here we present an easy-to-adopt simplified and shortened protocol.
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