Control of the stepwise assembly–disassembly of DNA origami nanoclusters by pH stimuli-responsive DNA triplexes

We present the pH-triggered reversible assembly of DNA origami clusters in a stepwise fashion. The structure formation and dissociation are controlled by a series of consecutive pH-stimulation processes that rely on the triplex-to-duplex transition of DNA triplexes in different pH conditions. This multilevel dynamic assembly strategy brings more structural complexity and provides the possibility of developing intelligent materials for engineering applications.