Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T

Amycolatopsis regifaucium is a Gram-positive actinomycete that produces polyphenol antibiotics such as kigamicins. As a member of the order Actinomycetales which represents a prolific source of biologically active compounds, the genome of this organism was sequenced to explore its biosynthetic potential. Here, we report a draft genome of Amycolatopsis regifaucium DSM 45072^T, comprising 8.28 Mb in genome size with 62 contigs, 312 subsystems, 7,966 coding genes, and 52 RNAs with the G+C content of 68.5 mol%. Genome mining studies revealed the presence of an unidentified glycopeptide antibiotic and a type II polyketide synthases (PKS) gene cluster in its genome. Further in-silico analysis, combined with wet lab experiments such as HPLC and LCMS, confirmed the production of decaplanin, previously reported from Amycolatopsis decaplanina DSM 44594^T, where its gene cluster was also identified. For the first time, a putative type II PKS gene cluster has been predicted within the glycopeptide antibiotic-producing clade (cluster A) of the genus Amycolatopsis, which is known for producing aromatic polyketide antibiotics, such as kigamicins.