伊卢森斯爱马仕
天蚕素
抗菌剂
生物
抗菌肽
微生物学
抗生素
细菌
抗生素耐药性
最小抑制浓度
遗传学
幼虫
植物
作者
Jian Peng,Lü Li,Yan Wu,Yifan Yang,Xiaoqin An,Kexin Yuan,Zhilang Qiu,Yinhui Jiang,Guo Guo,Feng Shen,Guiyou Liang
标识
DOI:10.1016/j.dci.2023.105111
摘要
Antimicrobial peptides are potential alternatives to traditional antibiotics in the face of increasing bacterial resistance. Insects possess many antimicrobial peptides and have become a valuable source of novel and highly effective antimicrobial peptides. Hermetia illucens as a resource insect, for example, has the highest number of antimicrobial peptides of any dipteran. However, most antimicrobial peptides, especially cecropin, have not been comprehensively identified and have not been evaluated for their antimicrobial ability. In this study, we analyzed the localization and gene structure of 33 cecropin molecules in the H. illucens genome and evaluated their activity against common human pathogens. The results showed that 32 cecropin molecules were concentrated on 1 chromosome, most with 2 exons. More importantly, most of the cecropins had a good antibacterial effect against Gram-negative bacteria, and were not hemolytic. The minimum inhibitory concentration (MIC) of the cecropin designated H3 against E. coli was 4 μg/mL. The toxicity, killing time kinetics, and anti-biofilm activity of H3 were further investigated and confirmed its antimicrobial ability. Overall, H3 is a potential candidate for the development of new antimicrobials to treat severe infections caused by Gram-negative pathogens such as E. coli.
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