Using Antibodies: A Laboratory Manual

Preface Dedication Chapter 1: Antibody Structure And Function Chapter 2: Antibody-Antigen Interactions Chapter 3: Choosing Antibodies Chapter 4: Handling Antibodies Storing Antibodies Purifying Antibodies Labeling Antibodies Chapter 5: Staining Cells Major Constraints Choosing The Correct Antibody Protocols For Immunostaining Of Tissue Culture Cells Variations On The Immunostaining Procedure Chapter 6: Staining Tissues Major Constraints Choosing the Correct Antibody Immunostaining Tissues With Polyclonal Or Monoclonal Antibodies Protocols For Staining Tissue Sections Variations For Staining Yeast Variations For Staining Drosophila Protocols For Antigen Retrieval Chapter 7: Immunoprecipitation Major Constraints Choosing The Correct Antibody Immunoprecipitation Protocols Variations On The Immunoprecipitation Procedure Chapter 8: Immunoblotting Major Constraints Choosing The Correct Antibody Immunoblotting Protocols Variations In The Detection Methods Variations In The Immunoblotting Technique Chapter 9: Immunoaffinity Purification Major Constraints Choosing The Correct Antibody Immunoaffinity Purification With Polyclonal And Monoclonal Antibodies Protocols For Immunoaffinity Purification Variations On Immunoaffinity Purification Chapter 10: Tagging Proteins Major Constraints Why Tag Your Protein Tags For Protein Detection Tags For Protein Purification Choosing The Right Tag Deciding Where To Position The Tag How To Tag Your Protein Chapter 11: Epitope Mapping Determining The Structural Requirements Of An Epitope Choosing An Epitope-mapping Method Mapping By Competition Assay Mapping By Expression Of Gene Fragments Mapping Using Synthetic Peptides Synthesis Or Purchase Of The Peptide Set Alternate Approaches To Epitope Mapping Appendix I: Electrophoresis Appendix II: Protein Techniques Appendix III: General Information Appendix IV: Cautions Appendix V: Trademarks Appendix VI: Suppliers