The effects of the biological dissolution and reduction of goethite on mercury methylation by Shewanella oneidensis MR-1 were studied under laboratory conditions. The results showed that S. oneidensis MR-1 could dissolve goethite, and subsequently dissolved Fe3+ which was reduced to Fe2+. S. oneidensis MR-1 was also a kind of mercury methylation bacterial, which had a potential to transform inorganic mercury to methylmercury. The biological dissolution and reduction of goethite declined with the increasing initial dosage of goethite. The dissimilatory reduction of goethite could promote mercury methylation to a certain extent. Weakly acidic condition was more beneficial to mercury methylation than neutral, alkaline or highly acidic conditions. Low humic acid concentration