Effect of wild KLF6 on prostate cancer cell line LNCaP

AIM:To observe the effect of wild type-KLF6(wt-KLF6) on proliferation,cell cycle,invasion and migration of prostate cancer cell line LNCaP by transgenic method.METHODS:Wt-KLF6 cDNA was obtained by RT-PCR method from liver cell and inserted into plasmid pEGFP-C1 for transfecting into LNCaP cells.The untransfected LNCaP cells served as the controls.MTT was used for observing the cell growth suppression,flow cytometer for apoptosis and cycle phase,invasion capability experiment for cell invasion capability with anti-oncogene wt-KLF6 on prostate cancer cell line LNCaP by transgenic method for 48 h.RESULTS:After wt-KLF6 was transfected into LNCaP cells,the growth suppression of LNCaP cells was enhanced [(31.9±4.7)% vs 0%,P0.01],the apoptosis was increased [(29.3±3.7)% vs(8.6±0.9)%,P0.01],the invasion capability was decreased[immigrated cell population/mm2,(102.8±15.4) vs(192.7±25.2),P0.05].Wt-KLF6 also decreased the ratio of LNCaP cell in phase G2/M,increased ratio of G0/G1 from control group(55.9±7.1)% to transfection group(75.0±8.8)%(P0.05).CONCLUSION:Wt-KLF6 transfection can suppress the growth and induce the apoptosis,decrease the invasive capability of LNCaP cells,probably by reversing partially its malignant phenotype.