A colorimetric Assay for Determination of Reactive Oxygen Produced by Activated Neutrophils with MTS

Objective To establish a simple,valid assay for measurement of respiratory burst in phagocytes.Methods The superoxide anions produced by those neutrophils pretreated with stimulators were detected through MTS as hydrogen receptor,then the respiratory burst in the cells was calculated indirectly.Results MTS colorimetric assay was correlative to ferric cytochrome C reduction assay significantly (Y _ (MTS) =2.307 X -0.291, r =0.994 1).The reactivc oxygen in 19 patients with recurrent infection was lower than normal ones significantly.Conclusions This assay is a simple,valid assay for measurement of respiratory burst in phagocytes,can be used in determination for phagocyte function.