The exams of sperm motility from 42 fertile men were carried out under 4 variant temperature of 17-37℃ to seek for affection of temperature on in vitro sperm motility. The results are as follows: The temperature evidently affects the grade of formard progression of the sperm and the percentage of motile sperm. The sperm forward progression is more sensitive to temperature changes than the percentage of motile sperm. In 36-37℃ group sperm motility is the highest Comparing the 36-37℃ group with the 24-25℃ group, there is no statistical differences in the grade of forward progression of the sperm (0-3 grade system)and in the percentage of motile sperm. If temperature is below 24℃, sperm motility will be slowed down. Authors suggest that the optimal temperature for routine exams of sperm motility should be carried out in 24℃ or over 24℃, while the optimal temperature for the experiment of reflecting the best sperm motility should be carried out in the left and right of 37℃.