As a beneficial bacteria,clostridium butyricum can produce a variety of vitamins,and has a health care on the human body.The spores of Clostridium butyricum are ideal microecological agents which have development prospects.Clostridium butyricum high-density culture must be carried out first before the preparation of microecological agents,followed by a higher conversion rate of butyric acid bacteria spores.Clostridium butyricum culture conditions were optimized in this paper and achieved good results.