Research Progress of Profound Hypothermia

Deep cryopreservation of cells and tissues has high clinical and research value.Controlled-rate freezing,one of the two key fields in cryopreservation techniques,is the first one to be developed.Cryoprotectants protect cells and tissues from freezing injury by increasing the solution viscosity and the freezing rate.Researches on the optimal Cryoprotectant provide the theory basis for preservation and clinical application of tissue-engineered products.Vitrification in recent years is getting more and more attention.Vitrification has the merits such as frozen fast,less freeze-thaw damage and easy operation.It can improve the survival rate after cryopreservation.In this review,the composition,classification,application and development of Cryoprotectant are demonstrated and the major strides and obstacles in cryopreservation will be reviewed.