Real‐Time Fluorescence Turn‐On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe

Abstract A tetracationic perylene probe (probe 2 ) was designed and synthesized. Probe 1 was used for the real‐time fluorescence turn‐on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.