蛋白酶
蛋白酵素
凝血酶
炭疽杆菌
劈理(地质)
化学
肽
胶体金
表面等离子共振
纳米颗粒
基质(水族馆)
酶
生物物理学
生物化学
色谱法
凝结
组合化学
纳米技术
材料科学
生物
细菌
血小板
复合材料
免疫学
精神科
遗传学
断裂(地质)
生态学
心理学
作者
Cristian Guarise,Lucia Pasquato,Vincenzo De Filippis,Paolo Scrimin
标识
DOI:10.1073/pnas.0509372103
摘要
We describe here a simple assay that allows the visual detection of a protease. The method takes advantage of the high molar absorptivity of the plasmon band of gold colloids and is based on the color change of their solution when treated with dithiols. We used C- and N-terminal cysteinyl derivatives of a peptide substrate exploiting its selective recognition and cleavage by a specific protease. Contrary to the native ones, cleaved peptides are unable to induce nanoparticles aggregation; hence, the color of the solution does not change. The detection of two proteases is reported: thrombin (involved in blood coagulation and thrombosis) and lethal factor (an enzyme component of the toxin produced by Bacillus anthracis ). The sensitivity of this nanoparticle-based assay is in the low nanomolar range.
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