Double-Stranded Universal Probe Strategy Enables Cost-Effective and Versatile Nucleic Acid Amplification Tests

copies/μL), with a limit of detection ranging from 10 copies/μL to 100 copies/μL. Furthermore, results from clinical samples showed high consistency and concordance with the gold standard qPCR, confirming the clinical viability and reliability of the ds-Uprobe-mediated assays. This simple, cost-effective, and highly versatile strategy enables sensitive and accurate detection and holds substantial promise for broader integration into various nucleic acid amplification tests for molecular diagnostics.