生物发光
体内
NAD+激酶
体外
化学
荧光素
荧光素酶
生物发光成像
生物化学
醌
氧化还原
生物物理学
生物
酶
转染
生物技术
基因
有机化学
作者
Yuhong Luo,Wentao Wang,Yi Zeng,Shuangqing Wang,Xudong Guo,Rui Hu,Guoqiang Yang
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2022-11-21
卷期号:147 (23): 5264-5268
被引量:1
摘要
NAD(P)H quinone oxidoreductase1 (NQO1) is a flavoenzyme that regulates the redox potential level in cells. Overexpression of NQO1 has been proven to be relevant to some malignant tumors. Herein a bioluminescent probe NQO1-Luc equipped with an NQO1-targeting group, trimethyl-locked quinone propionic acid (QPA), was constructed. The reduction of NQO1-Luc could be triggered by NQO1, resulting in the release of free D-luciferin, and concomitantly a bright bioluminescence emission. NQO1-Luc exhibits high selectivity and sensitivity toward NQO1 activity and the capability of distinguishing NQO1-overexpressing cells in vitro and in vivo, which offers a promising tool for investigations of NQO1 related biological processes including tumors in living organisms.
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