化学
色谱法
油酸
云芝
抗菌剂
紫色红曲霉
高效液相色谱法
红曲霉
食品科学
漆酶
生物化学
有机化学
发酵
酶
作者
Helen Smith,Seán Doyle,Richard F. Murphy
出处
期刊:Food Chemistry
[Elsevier]
日期:2023-03-01
卷期号:405: 134743-134743
被引量:3
标识
DOI:10.1016/j.foodchem.2022.134743
摘要
Some of the most powerful natural antimicrobial compounds originate from filamentous fungi. However, due to the diversity of compounds from plant and fungal origin, separation, isolation, and identification of bioactive constituents can be a long and tedious process. This study explores the effectiveness of thin layer chromatography (TLC) in combination with bioautography in the separation and identification of bioactive compounds from several filamentous fungi. Ultra-performance liquid chromatography coupled to photodiode array detector (UPLC-DAD) was employed to quantitatively identify phenolic composition. The total phenolic content of the selected filamentous fungi ranged from 31.85 mg g−1 to 101.77 mg g−1. Additionally, liquid chromatography mass spectrometry (LC/MS) determined the most abundant fatty acids were linoleic, palmitic, oleic, and stearic acid. Submerged cultivation of Grifola frondosa, Monascus purpureus, Lentinula edodes, Trametes versicolor and Pleurotus ostreatus proved to be an effective method to produce natural antimicrobial compounds.
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