Raman spectroscopy: Principles and recent applications in food safety

Food contaminant is a significant issue because of the adverse effects on human health and economy. Traditional detection methods such as liquid chromatography-mass spectroscopy for detecting food contaminants are expensive and time-consuming, and require highly-trained personnel and complicated sample pretreatment. Raman spectroscopy is an advanced analytical technique in a manner of non-destructive, rapid, cost-effective, and ultrasensitive sensing various hazards in agri-foods. In this chapter, we summarized the principle of Raman spectroscopy and surface enhanced Raman spectroscopy, the methods to process Raman spectra, the recent applications of Raman/SERS (surface-enhanced Raman spectroscopy) in detecting chemical contaminants (e.g., pesticides, antibiotics, mycotoxins, heavy metals, and food adulterants) and microbiological hazards (e.g., Salmonella, Campylobacter, Shiga toxigenic E. coli, Listeria, and Staphylococcus aureus) in foods.