“Two-in-one” core–shell nanozyme probes with double signal amplification for high-performing surface plasmon resonance immunosensing

Herein, a "two-in-one" Ag@Au core-shell nanozyme probe inducing double-signal amplification has been developed to significantly elevate the sensitivity of SPR sensors via sandwich immunoassay. The Ag@Au core-shell nanozyme with intrinsic peroxide-like activity was demonstrated to catalyze a polymerization reaction leading to formation of polyaniline, allowing further improvement of detection performance of SPR immunosensor. The method demonstrated here offers a universal strategy for enhanced SPR detection and further expands the application of nanozymes.