Quantification and Characterization of the Main Components of the Ethanolic Extract of Indian Cloves,Syzygium aromaticum[I] Merr. et Perry

The Indian clove, Syzygium aromaticum [L] Merr.et Perry, has been popularly used in the treatment of several diseases.A more recent use of this spice in Brazil is a homemade formulation, based on the ethanolic extraction of the dried flower buds of S. aromaticum, which has shown to be efficient as mosquitoes repellent.Here we evaluated, through the development of a GC analytical technique, the concentrations of the main components of the ethanolic extract of Indian cloves (eugenol and eugenyl acetate), and possible responsible for the repellent activity, in this homemade formulation.Our results corroborated the efficiency of this protocol to extract eugenol and eugenyl acetate and established the ideal conditions for extraction.