信使核糖核酸
转染
污渍
孵化
化学
分子生物学
蛋白质表达
体外
生物
生物化学
基因
作者
Xi Zhou,Huichang Bi,Jing Jin,Rongrong Deng,Meng-Jia Ying,Yongtao Wang,Min Huang
出处
期刊:PubMed
日期:2013-05-01
卷期号:48 (5): 794-8
摘要
This study is purposed to investigate the effects of praeruptorin A (PA) and praeruptorin C (PC) on UGT1A1 in HepG2 cells through hCAR pathway. PA and PC were incubated with HepG2 cells for 24 h and 48 h, mRNA and protein expressions of UGT1A1 were determined by real-time PCR and Western blotting assays. Additionally, effects of PA and PC on UGT1A1 mRNA and protein expressions were also measured after transient transfection of a specific CAR siRNA for 72 h in HepG2 cells. UGT1A1 mRNA and protein expression levels were significantly increased by PA and PC after incubation for 48 h. Moreover, the mRNA and protein up-regulations of UGT1A1 were attenuated by transient transfection of a specific CAR siRNA, suggesting the induction was mediated by CAR. The results suggest that PA and PC can significantly up-regulate UGT1A1 expression partially via the CAR-mediated pathway.
科研通智能强力驱动
Strongly Powered by AbleSci AI