蛹虫草
一氧化氮
Toll样受体
肿瘤坏死因子α
巨噬细胞
MAPK/ERK通路
生物
分泌物
受体
多糖
细胞因子
细胞外
细胞生物学
先天免疫系统
免疫系统
信号转导
生物化学
化学
免疫学
冬虫夏草
体外
内分泌学
食品科学
作者
Jong Seok Lee,Duck Soo Kwon,Ki Rim Lee,Jun Myoung Park,Suk‐Jin Ha,Eock Kee Hong
标识
DOI:10.1016/j.carbpol.2014.11.059
摘要
Mushroom-derived polysaccharides have been shown to stimulate immune responses. Our previous report showed that the novel polysaccharide PLCM isolated from the culture broth of Cordyceps militaris could induce nitric oxide production in the murine macrophage-like cell line RAW264.7. In this study, we show that PLCM enhances immunostimulatory activities such as the release of toxic molecules (nitric oxide and reactive oxygen species), secretion of the cytokine tumor necrosis factor (TNF)-α, and phagocytic uptake in RAW264.7 macrophages. In addition, all the specific inhibitors against the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) (SN50, BAY11-7082, PD98059, SP600125 and SB203580) markedly suppressed the nitric oxide production and phagocytic uptake induced by PLCM. Moreover, antibodies specific to the extracellular domain of Toll-like receptor-2, Toll-like receptor-4 or the macrophage receptor Dectin-1 significantly attenuated PLCM-induced secretion of TNF-α. Our results indicate that the C. militaris polysaccharide activates macrophages through the MAPKs and NF-κB signaling pathways via Toll-like receptor 2, Toll-like receptor 4, and Dectin-1.
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