内输蛋白
生物
细胞生物学
核定位序列
核运输
生殖细胞
核蛋白
遗传学
细胞核
转录因子
细胞质
基因
作者
Arash Arjomand,Mark A. Baker,Chen Li,Ashley M. Buckle,David A. Jans,Kate Loveland,Yoichi Miyamoto
摘要
Importin α proteins function as adaptors to connect a cargo protein and importin β1 in the classical nuclear import pathway. Here we measure for the first time the stoichiometry of importins α2, α3, α4, and β1 in primary cells corresponding to 2 successive stages of rat spermatogenesis: meiotic spermatocytes and haploid round spermatids. Importin α2 levels were more than 2-fold higher in spermatocytes than in spermatids, while importins α4 and β1 levels did not differ significantly. We performed a comprehensive proteomics analysis to identify binding proteins in spermatocytes and spermatids using recombinant importin α2 and α4 proteins. Among the 100 candidate partners, 42 contained a strong classical nuclear localization signal (cNLS; score of>6 by cNLS Mapper), while 8 nuclear proteins lacked any cNLS. In addition, we developed a new strategy to predict which cargoes bind to importin α through the conserved C-terminal acidic domain (ARM repeats 9-10), and provided functional validation of a predicted importin α C-terminal binding segment in Senataxin and Smarca4. Evaluation of this set of candidate binding partners from spermatogenic cells using several bioinformatics approaches provides new evidence that individual importin αs may serve unique and nonredundant roles in mediating cellular differentiation.—Arjomand, A., Baker, M. A., Li, C., Buckle, A. M., Jans, D. A., Loveland, K. L., Miyamoto, Y. The α-importome of mammalian germ cell maturation provides novel insights for importin biology. FASEB J. 28, 3480–3493 (2014). www.fasebj.org
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