染色质
增强子
转录因子
拟南芥
细胞命运测定
遗传学
地图集(解剖学)
同源异型基因
计算生物学
表观遗传学
生物
调节顺序
计算机科学
染色体构象捕获
分辨率(逻辑)
细胞
单细胞分析
细胞生物学
高分辨率
电池类型
基因调控网络
基因
基因组
转录组
基因表达
DNA甲基化
突变体
作者
Alexandre P. Marand,Zongliang Chen,Andrea Gallavotti,Robert J. Schmitz
标识
DOI:10.1101/2020.09.27.315499
摘要
SUMMARY Cis -regulatory elements (CREs) encode the genomic blueprints of spatiotemporal gene expression programs enabling highly specialized cell functions. To identify CREs at cell-type resolution in Zea mays , we implemented single-cell sequencing of Assay for Transposase Accessible Chromatin (scATAC-seq) in seedlings, embryonic roots, crown roots, axillary buds, and pistillate and staminate inflorescence. We describe 92 states of chromatin accessibility across 165,913 putative CREs and 52 known cell types. Patterns of transcription factor (TF) motif accessibility predicted cell identity with high accuracy, uncovered putative non-cell autonomous TFs, and revealed TF motifs underlying higher-order chromatin interactions. Comparison of maize and Arabidopsis thaliana developmental trajectories identified TF motifs with conserved patterns of accessibility. Cell type-specific CREs were enriched with enhancer activity, phenotype-associated genetic variants, and signatures of breeding-era selection. These data, along with companion software, Socrates , afford a comprehensive framework for understanding cellular heterogeneity, evolution, and cis -regulatory grammar of cell-type specification in a major crop.
科研通智能强力驱动
Strongly Powered by AbleSci AI