The screening of the natural enemies of Monochamus alternatus is the basic work for studying the biological control of pine wood nematode disease.The dead pine wood in different periods were anatomized and observed to collect the subcutaneous insects and the insects within pine wood.The insects that could not be determined as trunk-boring pests were fed with Monochamus alternatus larvae.The parasitic natural enemies of Monochamus alternatus larvae,Dastarcus helcphoroide,and six kinds of feeding natural enemies of Monochamus alternatus larvae were found.And Dastarcus helcphoroide was first found in Guizhou.