定量蛋白质组学
蛋白质组学
质谱法
翻译后修饰
等压标记
化学
定量分析(化学)
串联质量标签
自下而上蛋白质组学
计算生物学
蛋白质质谱法
串联质谱法
色谱法
生物
生物化学
基因
酶
作者
Rune Matthiesen,Ana Sofía Carvalho
出处
期刊:Methods in molecular biology
日期:2009-11-06
卷期号:: 187-204
被引量:15
标识
DOI:10.1007/978-1-60327-194-3_10
摘要
Protein quantitation by mass spectrometry (MS) is attractive since it is possible to obtain both the identification and quantitative values of novel proteins and their posttranslational modifications in one experiment. In contrast, protein arrays only provide quantitative values of targeted proteins and their modifications. There are an overwhelming number of quantitative mass spectrometry (MS) methods for protein and peptide quantitation. The aim here is to provide an overview of the most common MS-based quantitative methods used in the proteomics field and discuss the computational algorithms needed for the robust quantitation of proteins, peptides, and their posttranslational modifications.
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