Differential analysis of two-dimensional gel electrophoresis profiles for oral lichen planus

Objective : To establish the two-dimensional Gel electrophoresis profiles with high resolution and reproducibility from human oral lichen planus and paired normal oral mucosa tissues ,and identify the differential expression proteins. Method : The total proteins of oral lichen planus and paired normal oral mucosa tissues were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).After AgNO3 staining,the differential expression proteins were ana1yzed by using ImageMaster image ana1ysis software. Result :(1)The well-resolved,reproducible 2-DE patterns of oral lichen planus and normal oral mucosa tissues were obtained. The results showed that average spots were 1576±67, 1608±73 in oral lichen planus and normal oral mucosa tissues respectively.(2) The 13 differential protein spots were identified by Image Master image analysis software. There were 7 protein spots in oral lichen planus were higher expression than that in normal oral mucosa. 6 protein spots in oral lichen planus were lower expression than that in normal oral mucosa. Conclusion : In this study, the well-resolved reproducible 2-DE patterns of oral lichen planus and normal oral mucosa tissues were established and some differential proteins were characterized.These data will be further used to screen special biomarkers of oral lichen planus .