Preparation of Hydroxybutyl Chitosan and Its Biocompatibility Evaluation with Mouse Embryonic Fibroblasts

Objective To prepare and study the biocompatibility of Hydroxybutyl chitosan(HBC) with mouse embryonic fibroblasts(MEF) in vitro.To investigate the feasibility of using HBC as tissue engineering materials.Methods The HBC were prepared by chitosan chemically modified and the best preparation conditions were summarized.Different solvents were used on HBC for the solubility test.The structure of the HBC was characterized by IR and scanning electron microscopy(SEM).MEFs were isolated and cultivated from mouse embryo.The biocompatibility of HBC is investigated by using the MTT assay.Results All the four HBC samples could dissolve in water and HBC 4 which was acted 14 hours at 55 ℃ have better water-solubility.HBC solution can rapidly transform to semisolid at body temperature(37 ℃) and revert to its solvated state by physical cross-liking at lower temperature.MEF cultured with four kinds of leaching of HBC were investigated by the MTT assay and the cytotoxicity of HBC was in graded 0 and 1.Conclusion HBC was prepared successfully with good thermo-sensitivity and could be transformed into gelation at body temperature(37 ℃).HBC presented good biocompatibility with MEFs and could be used as tissue engineering materials.