核酸
纳米团簇
冷凝
DNA
核糖核酸
化学
疏水效应
生物物理学
纳米技术
缩合反应
DNA缩合
材料科学
有机化学
生物化学
生物
基因
催化作用
转染
物理
热力学
作者
Yu Liu,Haoran Zheng,Hui Liu,Mingxing Duan,Cong Li,Mingqiang Li,Li Jiang,Lihua Wang,Qian Li,Jing Chen,Jianlei Shen
出处
期刊:JACS Au
[American Chemical Society]
日期:2023-08-07
卷期号:3 (8): 2206-2215
被引量:1
标识
DOI:10.1021/jacsau.3c00252
摘要
Nucleic acid condensates are essential for various biological processes and have numerous applications in nucleic acid nanotechnology, gene therapy, and mRNA vaccines. However, unlike the in vivo condensation that is dependent on motor proteins, the in vitro condensation efficiency remains to be improved. Here, we proposed a hydrophobic interaction-driven mechanism for condensing long nucleic acid chains using atomically precise hydrophobic gold nanoclusters (Au NCs). We found that hydrophobic Au NCs could condense long single-stranded DNA or RNA to form composites of spherical nanostructures, which further assembled into bead-shaped suprastructures in the presence of excessive Au NCs. Thus, suprastructures displayed gel-like behaviors, and Au NCs could diffuse freely inside the condensates, which resemble the collective motions of condensin complexes inside chromosomes. The dynamic hydrophobic interactions between Au NCs and bases allow for the reversible release of nucleic acids in the presence of mild triggering agents. Our method represents a significant advancement toward the development of more efficient and versatile nucleic acid condensation techniques.
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