清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Rheum officinale and Salvia miltiorrhiza inhibit renal fibrosis via miR-21/PTEN/Akt signaling pathway in vitro and in vivo

PTEN公司 蛋白激酶B 丹参 张力素 医学 纤维化 PI3K/AKT/mTOR通路 骨桥蛋白 污渍 癌症研究 信号转导 病理 化学 内科学 中医药 生物化学 替代医学 基因
作者
Hong Wang,Qian Jiang,Li Kang,Yuan Ling,Guan Chen,Xiaoxue Cui,Luyao Wang,Ting Zhang,Lei Wang
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:304: 115928-115928 被引量:6
标识
DOI:10.1016/j.jep.2022.115928
摘要

As one of the main components of many famous Chinese herbal formulas, Rheum palmatum L. and Salvia miltiorhiza Bunge (RS) are extensively used to treat chronic kidney disease (CKD). RS has been proved to improve renal function and relieve renal fibrosis (RF), but the potential mechanism remains a mystery.The purpose of this study is to determine whether microRNA-21 (miR-21) is associated with RF progression, as well as whether RS protects against RF through miR-21/PTEN/AKT signaling.(1) The rat model of RF was established using unilateral ureteral obstruction (UUO). After UUO surgery, miR-21 levels in plasma were detected by RT-PCR and RF scores were assessed by Masson's trichrome stain at days 3, 7, 14 and 21. The correlation analysis of the above two indexes was carried out by Spearman correlation analysis. (2) Human proximal tubular epithelial cells (HK-2) was transfected with miR-21 mimic and inhibitor, and then the levels of phosphatase and tensin homolog (PTEN) protein and mRNA were measured with Western blotting and RT-PCR, respectively. (3) TGF-β (10 ng/mL) was added into HK-2 cells to induce fibrosis, followed by the intervention of RS-containing rat serum. PTEN and protein kinase-B (Akt) phosphorylation, as well as the expression of PTEN protein in HK-2 cells, were assessed by RT-PCR, Western blotting and immunofluorescence. (4) The rat models of RF were prepared by UUO and treated with RS. Serum creatinine and urea nitrogen levels were measured. RF score was determined by Masson's trichrome stain. RT-PCR was used to determine the expression of miR-21, PTEN, and Akt mRNA. Western blotting was used to determine the expression of PTEN and Akt proteins.A positive correlation was found between plasma miR-21 levels and RF scores of rats after UUO surgery at Days 3, 7, 14 and 21. It was confirmed that miR-21 targeted PTEN. RS drug-containing serum could rise the expression of PTEN and reduce Akt phosphorylation of HK-2 cells induced by TGF-β. Moreover, RS drug-containing serum could increase PTEN expression and reduce Akt phosphorylation induced by miR-21 mimic in HK-2 cells. The rats treated with RS had significantly decreased serum creatinine and urea nitrogen levels and a lower RF score. RS also decreased miR-21 and Akt expressions, increased PTEN expression of UUO rats.There was a positive correlation between plasma miR-21 levels and RF scores. The inhibitory effect of RS on RF might be mediated by miR-21/PTEN/AKT signaling.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
依居发布了新的文献求助10
4秒前
蓝胖胖蓝完成签到,获得积分10
10秒前
30秒前
稀松完成签到,获得积分10
31秒前
34秒前
陌子完成签到 ,获得积分10
38秒前
zjq完成签到 ,获得积分10
45秒前
51秒前
飞云完成签到 ,获得积分10
1分钟前
铁妹儿完成签到 ,获得积分10
1分钟前
SHD完成签到 ,获得积分10
1分钟前
煜琪完成签到 ,获得积分10
1分钟前
高贵逍遥完成签到 ,获得积分10
1分钟前
wyh295352318完成签到 ,获得积分10
2分钟前
jie完成签到 ,获得积分10
2分钟前
さくま完成签到,获得积分10
2分钟前
啥时候能早睡完成签到 ,获得积分10
2分钟前
dbdxyty完成签到,获得积分10
2分钟前
沉香如兰完成签到,获得积分10
2分钟前
幸福安康完成签到 ,获得积分10
2分钟前
mark33442完成签到,获得积分10
2分钟前
qq完成签到 ,获得积分10
2分钟前
潇潇雨歇完成签到,获得积分10
2分钟前
2分钟前
2分钟前
洁净的盼易完成签到 ,获得积分10
3分钟前
枫林摇曳完成签到 ,获得积分10
3分钟前
葫芦芦芦完成签到 ,获得积分10
3分钟前
魔幻的慕梅完成签到 ,获得积分10
3分钟前
成就的笑南完成签到 ,获得积分10
3分钟前
wujiwuhui完成签到 ,获得积分10
3分钟前
温婉的人雄完成签到,获得积分10
3分钟前
xzy998应助依居采纳,获得10
3分钟前
zz完成签到 ,获得积分10
4分钟前
依居完成签到,获得积分10
4分钟前
MISA完成签到 ,获得积分10
4分钟前
loga80完成签到,获得积分0
4分钟前
4分钟前
中中中发布了新的文献求助10
4分钟前
XKINGLEE完成签到 ,获得积分10
4分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052630
求助须知:如何正确求助?哪些是违规求助? 2709863
关于积分的说明 7418232
捐赠科研通 2354395
什么是DOI,文献DOI怎么找? 1246007
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921