结直肠癌
癌症研究
癌症干细胞
下调和上调
干细胞
细胞
大肠癌小鼠模型的建立
癌细胞
细胞凋亡
化学
活力测定
细胞生物学
癌症
细胞生长
程序性细胞死亡
医学
生物
内科学
生物化学
基因
作者
Jie Sun,Xiangjun Cheng,Shubo Pan,Liangjing Wang,Wenhuan Dou,Jie Liu,Xiaohua Shi
摘要
Abstract Colorectal cancer stem cell (CSC) has been regarded to be the root of colorectal cancer progression. However, there is still no effective therapeutic method targeting colorectal CSC in clinical application. Here, we investigated the effects of dichloroacetate (DCA) on colorectal cancer cell stemness. We showed that DCA could reduce colorectal cancer cell stemness in a dose‐dependent manner, which is evident by the decreased expression of stemness markers, tumor cell sphere‐formation and cell migration ability. In addition, it was found that DCA trigerred the ferroptosis of colorectal CSC, which is characterized as the upregulation of iron concentration, lipid peroxides, and glutathione level, and decreased cell viability. Mechanistic studies demonstrated that DCA could sequester iron in lysosome and thus trigger ferroptosis, which is necessary for DCA‐mediated attenuation on colorectal cancer cell stemness. Taken together, this work suggests that DCA might be a colorectal CSC‐killer.
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