巨噬细胞
刚度
基质(化学分析)
细胞生物学
材料科学
复合材料
化学
生物
生物化学
体外
出处
期刊:Methods in molecular biology
日期:2020-08-18
卷期号:: 111-120
被引量:4
标识
DOI:10.1007/978-1-0716-0845-6_11
摘要
The immune system depends on two major paths—the innate and the adaptive immunity. Macrophage, with its unique features as the first line of immune defense to engulf and digest invaders, serves as the key effector cells integrating those two paths. The dynamic plasticity of macrophage activation during wound repair, inflammation resolution, and tissue remodeling are emerging biomedical and bioengineering hot topics in immune function studies such as the various secretions of cytokines and chemokines and the signaling pathways with ligands and their cognate receptors. Better knowledge on how physical/mechanical and multicellular microenvironment on the modulation of macrophage functions will create innovative therapies to boost host defense mechanism and assist wound healing. In this, we describe an easy method to measure functions (gene expressions) of human and mouse macrophages in response to mechanical microenvironment changes by embedding isolated macrophages in polymerized hyaluronan gel with different wound matrix stiffness.
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