Rational Design of Helix‐Stabilized Antimicrobial Peptide Foldamers Containing α,α‐Disubstituted Amino Acids or Side‐Chain Stapling

Abstract Antimicrobial peptides (AMPs) are expected to be good candidate molecules for novel antimicrobial therapies. Most AMPs exert their antimicrobial activity through disruption of microbial membranes due to their amphipathic properties. Recently, the helical peptide ‘Stripe’ was reported by our group, a rationally designed amphipathic AMP focused on distribution of natural cationic and hydrophobic amino acid residues. In this study, a set of Stripe ‐based AMP foldamers was designed, synthesized and investigated that contain α,α‐disubstituted amino acids or side‐chain stapling to stabilize their helical structures. Our results showed that a peptide containing 2‐aminoisobutyric acid (Aib) residues exhibited potent antimicrobial activity against both Gram‐positive S.aureus (MIC value: 3.125 μM) and Gram‐negative bacteria (including a multidrug‐resistant strain, MDRP, MIC value: 1.56 μM), without significant hemolytic activity (>100 μM). Electrophysiological measurements revealed that this peptide formed stable pores in a 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine (DOPE)/1,2‐dioleoyl‐sn‐glycero‐3‐phosphoglycerol (DOPG) bilayer but not in a dioleoylphosphocholine (DOPC) bilayer. The introduction of Aib residues into Stripe could be a promising way to increase the antimicrobial activity of AMP foldamers, and the peptide could represent a promising novel therapeutic candidate to treat multidrug‐resistant bacterial infection.