病毒载量
环介导等温扩增
人类免疫缺陷病毒(HIV)
人口
宫颈癌
病毒学
人乳头瘤病毒
医学
检测点注意事项
计算生物学
癌症
免疫学
生物
DNA
内科学
环境卫生
遗传学
作者
Marjon Zamani,James Robson,Andy Fan,Michael S. Bono,Ariel L. Furst,Catherine M. Klapperich
出处
期刊:ACS central science
[American Chemical Society]
日期:2021-05-12
卷期号:7 (6): 963-972
被引量:42
标识
DOI:10.1021/acscentsci.1c00186
摘要
Sexually transmitted infections, including the human immunodeficiency virus (HIV) and the human papillomavirus (HPV), disproportionally impact those in low-resource settings. Early diagnosis is essential for managing HIV. Similarly, HPV causes nearly all cases of cervical cancer, the majority (90%) of which occur in low-resource settings. Importantly, infection with HPV is six times more likely to progress to cervical cancer in women who are HIV-positive. An inexpensive, adaptable point-of-care test for viral infections would make screening for these viruses more accessible to a broader set of the population. Here, we report a novel, cost-effective electrochemical platform using gold leaf electrodes to detect clinically relevant viral loads. We have combined this platform with loop-mediated isothermal amplification and a CRISPR-based recognition assay to detect HPV. Lower limits of detection were demonstrated down to 104 total copies of input nucleic acids, which is a clinically relevant viral load for HPV DNA. Further, proof-of-concept experiments with cervical swab samples, extracted using standard extraction protocols, demonstrated that the strategy is extendable to complex human samples. This adaptable technology could be applied to detect any viral infection rapidly and cost-effectively.
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