促炎细胞因子
益生菌
免疫系统
骨愈合
某种肠道细菌
医学
免疫学
变形链球菌
牙周纤维
细胞因子
下调和上调
微生物学
生物膜
炎症
伤口愈合
白细胞介素
脚手架
牙周炎
分泌物
骨感染
作者
Shiyuan Song,Wen Zhang,Hongmei Zhuang,Wei Wei,Shuyu Cheng,Dan Qiao,Yin Xiao,Yangheng Zhang,Fuhua Yan,Shiyuan Song,Wen Zhang,Hongmei Zhuang,Wei Wei,Shuyu Cheng,Dan Qiao,Yin Xiao,Yangheng Zhang,Fuhua Yan
标识
DOI:10.1016/j.bioactmat.2025.11.019
摘要
Oral and maxillofacial bone defects are prevalent and challenging to treat, often leading to significant complications such as infection and impaired healing. Enhancing the antibacterial and osteoimmunomodulatory properties of bone graft materials represents a promising approach to improve regenerative outcomes. In this study, we developed a hydroxyapatite scaffold coated with a biofilm of the probiotic Akkermansia muciniphila (Akk-HA), engineered to simultaneously combat infection and modulate the immune environment. Akk-HA exhibited vigorous anti-adhesive activity against pathogenic bacteria and attenuated inflammatory responses by suppressing proinflammatory cytokine secretion while promoting the release of proregenerative mediators from macrophages. Mechanistic studies revealed that Akk-HA activated the PI3K/AKT signalling pathway, leading to the upregulation of interleukin-10 (IL-10), in turn enhancing the osteogenic differentiation of periodontal ligament cells (PDLCs). In a murine model of infected periodontal bone defects, Akk-HA demonstrated significant antibacterial and immunomodulatory effects, resulting in markedly improved bone regeneration. These findings highlight the therapeutic potential of probiotic-functionalized bone grafts as a dual-action strategy for managing infected bone defects in the oral and maxillofacial regions.
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