厚壁菌
微生物群
代谢组
生物
蛋白质组
消化(炼金术)
抗性淀粉
代谢组学
肠道菌群
拟杆菌
膳食纤维
基因组
食品科学
肠道微生物群
细菌
淀粉
微生物代谢
生物化学
化学
生物信息学
遗传学
基因
16S核糖体RNA
色谱法
作者
Tanja Maier,Marianna Lucio,Lang Ho Lee,Nathan C. VerBerkmoes,Colin Brislawn,Jörg Bernhardt,Regina Lamendella,Jason McDermott,Nathalie Bergeron,Silke S. Heinzmann,James T. Morton,Antonio González,Gail Ackermann,Rob Knight,Katharina Riedel,Ronald M. Krauss,Philippe Schmitt‐Kopplin,Janet Jansson
出处
期刊:MBio
[American Society for Microbiology]
日期:2017-11-08
卷期号:8 (5)
被引量:214
标识
DOI:10.1128/mbio.01343-17
摘要
ABSTRACT Diet can influence the composition of the human microbiome, and yet relatively few dietary ingredients have been systematically investigated with respect to their impact on the functional potential of the microbiome. Dietary resistant starch (RS) has been shown to have health benefits, but we lack a mechanistic understanding of the metabolic processes that occur in the gut during digestion of RS. Here, we collected samples during a dietary crossover study with diets containing large or small amounts of RS. We determined the impact of RS on the gut microbiome and metabolic pathways in the gut, using a combination of “omics” approaches, including 16S rRNA gene sequencing, metaproteomics, and metabolomics. This multiomics approach captured changes in the abundance of specific bacterial species, proteins, and metabolites after a diet high in resistant starch (HRS), providing key insights into the influence of dietary interventions on the gut microbiome. The combined data showed that a high-RS diet caused an increase in the ratio of Firmicutes to Bacteroidetes , including increases in relative abundances of some specific members of the Firmicutes and concurrent increases in enzymatic pathways and metabolites involved in lipid metabolism in the gut. IMPORTANCE This work was undertaken to obtain a mechanistic understanding of the complex interplay between diet and the microorganisms residing in the intestine. Although it is known that gut microbes play a key role in digestion of the food that we consume, the specific contributions of different microorganisms are not well understood. In addition, the metabolic pathways and resultant products of metabolism during digestion are highly complex. To address these knowledge gaps, we used a combination of molecular approaches to determine the identities of the microorganisms in the gut during digestion of dietary starch as well as the metabolic pathways that they carry out. Together, these data provide a more complete picture of the function of the gut microbiome in digestion, including links between an RS diet and lipid metabolism and novel linkages between specific gut microbes and their metabolites and proteins produced in the gut.
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