爆发
医学
百日咳博德特菌
聚合酶链反应
攻击率
23S核糖体RNA
病毒学
儿科
生物
基因
遗传学
细菌
核糖核酸
核糖体
作者
Xiaoguai Liu,Zengguo Wang,Juansheng Zhang,Fang Li,Yang Luan,Hao Li,Yarong Li,Qiushui He
出处
期刊:Pediatric Infectious Disease Journal
[Ovid Technologies (Wolters Kluwer)]
日期:2018-06-01
卷期号:37 (6): e145-e148
被引量:25
标识
DOI:10.1097/inf.0000000000001814
摘要
Background: A pertussis outbreak was studied in a primary school in Xi’an, China, in March 2016. The school consisted of 536 pupils 6–12 years of age who were divided into 12 classes of 6 grades (2 classes for each grade). The identified index case was an 11-year-old girl at class 2 of grade 5. Methods: Interview was conducted and nasopharyngeal swabs were taken from all pupils (N = 94) in the 2 classes of grade 5. Nasopharyngeal swabs were tested by both culture and polymerase chain reaction (PCR). Results: Four culture- and 17 PCR-positive cases were identified in 94 pupils. Infection rate was significantly higher in class 2 compared with that in class 1 [37.0% (17/46) vs. 14.6% (7/48), χ 2 = 4.26, P < 0.05]. All Bordetella pertussis isolates were macrolide-resistant, harbored prn1 / ptxP1 / fim3-1 as previously reported and belonged to multilocus variable tandem repeat analysis type MLVA 195. Of the 17 DNAs positive for diagnostic PCR, 12 were also positive for 23S ribosomal RNA PCR. All the 12 DNAs had the A2047G mutation of 23S rRNA gene of B. pertussis . Conclusions: This study described a pertussis outbreak caused by macrolide-resistant B. pertussis in a primary school and indicated that close contact of index case causes the bacterial transmission.
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