清脆的
棉铃虫
生物
Cas9
索引
遗传学
基因组
外显子
亚基因组mRNA
基因组编辑
基因
回文
计算生物学
基因型
单核苷酸多态性
植物
生殖器鳞翅目
作者
Minghui Jin,Yutao Xiao,Ying Cheng,Jie Hu,Chao‐Bin Xue,Kongming Wu
出处
期刊:Insect Science
[Wiley]
日期:2018-02-28
卷期号:26 (6): 1029-1036
被引量:14
标识
DOI:10.1111/1744-7917.12570
摘要
Abstract Helicoverpa armigera , cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2 . Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera .
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