化学
卵巢癌
卵巢癌
发光
检出限
背景(考古学)
细胞培养
卵巢
发光测量
生物标志物
癌
癌症研究
癌症
生物化学
病理
内科学
光电子学
内分泌学
色谱法
生物
遗传学
物理
古生物学
医学
作者
Wanhe Wang,Kasipandi Vellaisamy,Guodong Li,Cheng‐Shyong Wu,Chung-Nga Ko,Chung‐Hang Leung
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2017-10-12
卷期号:89 (21): 11679-11684
被引量:133
标识
DOI:10.1021/acs.analchem.7b03114
摘要
β-Galactosidase (β-gal) is an important biomarker for ovarian cancers. In this work, we designed and synthesized a novel iridium(III)-based probe 1 for discriminating ovarian carcinoma cell lines from normal cell lines. The probe could detect β-gal even in the presence of a highly autofluorescent background. The probe also showed a good linear response to β-gal between 0 and 30 U/mL, with a detection limit of 0.51 U/mL. Importantly, complex 1 could selectively “light up” ovarian carcinoma cells, while exhibiting negligible luminescence in normal cells. Overall, complex 1 could be potentially used as a useful probe for detecting β-gal expression in the context of ovarian cancer diagnostics.
科研通智能强力驱动
Strongly Powered by AbleSci AI