Phase-separation property of polyethylene glycol (PEG) or IgG conjugated PEG with dextran in microchannel

Polyethylene glycol (PEG) conjugated biomolecules, such as proteins, antibody fragments, aptamers, peptides, and other small molecules, are widely used, because PEG can increase the solubility and stability of conjugated biomolecules in general. The chemical attachment of PEG to these biomolecules is referred to as “PEGylation. Various PEGylation techniques are well established so far, especially the reaction conditions, desired site and degree of PEGylation, etc. In this study, phase-separation properties of PEG or PEGylated IgG with dextransolution was compared. PEG (M.W. 10000) or PEGylated (M.W. 10000) mouse anti-human IgG was used with dextran T500 (10%, w/w) solution. The volume ratio of two phases in microchannel was compared based upon the infused volumetric flow rate of each polymer solution. Developed technique will be used for the simultaneous phase-separation and affinity separation of biomolecues.