生物膜
赖氨酸
生物化学
变形链球菌
细菌
细胞外
酶
多糖
生物
代谢途径
化学
糖
微生物学
氨基酸
遗传学
作者
Zhengyi Li,Tao Gong,Qinrui Wu,Yixin Zhang,Xin Zheng,Yuqing Li,Biao Ren,Xian Peng,Xuedong Zhou
出处
期刊:Science Signaling
[American Association for the Advancement of Science (AAAS)]
日期:2023-09-05
卷期号:16 (801)
被引量:1
标识
DOI:10.1126/scisignal.adg1849
摘要
In eukaryotes, lactate produced during glycolysis is involved in regulating multiple metabolic processes through lysine lactylation (Kla). To explore the potential link between metabolism and Kla in prokaryotes, we investigated the distribution of Kla in the cariogenic bacterium Streptococcus mutans during planktonic growth in low-sugar conditions and in biofilm-promoting, high-sugar conditions. We identified 1869 Kla sites in 469 proteins under these two conditions, with the biofilm growth state showing a greater number of lactylated sites and proteins. Although high sugar increased Kla globally, it reduced lactylation of RNA polymerase subunit α (RpoA) at Lys 173 . Lactylation at this residue inhibited the synthesis of extracellular polysaccharides, a major constituent of the cariogenic biofilm. The Gcn5-related N-acetyltransferase (GNAT) superfamily enzyme GNAT13 exhibited lysine lactyltransferase activity in cells and lactylated Lys 173 in RpoA in vitro. Either GNAT13 overexpression or lactylation of Lys 173 in RpoA inhibited biofilm formation. These results provide an overview of the distribution and potential functions of Kla and improve our understanding of the role of lactate in the metabolic regulation of prokaryotes.
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