微透析
体内
免疫分析
纹状体
同种类的
生物信息学
化学
神经科学
药理学
帕金森病
生物
医学
多巴胺
内科学
生物化学
疾病
免疫学
抗体
物理
生物技术
基因
热力学
作者
Youngsoo Kim,Lequn Geng,Ashley E. Lenhart,Jay Y. Li,William T. Dauer,Robert T. Kennedy
标识
DOI:10.1021/acschemneuro.2c00251
摘要
Understanding the regulation of α-synuclein release could be important in better understanding Parkinson's disease development, progression, and treatment. Advances in such studies are hindered by technical challenges that limit the ability to monitor α-synuclein concentration in vivo. We developed a novel α-synuclein microdialysis method coupled with a specific and sensitive immunoassay that requires a small sample volume (1 μL). Using this method, basal α-synuclein level was estimated at 254 ± 78 pM in the striatum of freely moving mice. Additionally, we observed that potassium (75 mM) and nicotine (0.5 mg/kg) administration significantly increased α-synuclein in dialysates. These results provide evidence that the methods we report here can be useful to investigate the physiological roles of α-synuclein and support the idea that α-synuclein is secreted to the extracellular space in a neuronal activity-dependent manner.
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