永生化细胞系
生精小管
间充质干细胞
软骨发生
移植
脂肪组织
脂肪生成
生物
干细胞
细胞生物学
免疫学
内分泌学
内科学
细胞
支持细胞
医学
精子发生
遗传学
作者
Jia You Fang,Yudong Wei,Xin Teng,Shanting Zhao,Jinlian Hua
摘要
Abstract Adipose‐derived mesenchymal stem cells (ADSCs) are proven to provide good effects in numerous tissue engineering application and other cell‐based therapies. However, the difficulty in the proliferation of ADSCs, known as the “Hayflick limit” in vitro, limits their clinical application. Here, we immortalized canine ADSCs (cADSCs) with SV40 gene and transplanted them into busulfan‐induced seminiferous tubules of infertile mice. The proliferation of these immortalized cells was improved significantly. Then, cellular differentiation assays showed that the immortalized cADSCs could differentiate into three‐germ‐layer cells, osteogenesis, chondrogenesis, adipogenesis phenotypes, and primordial germ cell‐like cells (PGCLCs). In addition, the immortalized cADSCs can proliferate in the busulfan‐induced seminiferous tubules of infertile mice. These findings confirmed that the immortalized cADSCs maintain the criteria of cADSCs.
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